Imizekelo ye-Centrifugation

Ividiyo: Centrifugation| Separation Methods | Physics

Umxholo

Iindidi zecentrifugation
Imizekelo ye-centrifugation

Inkqubo ye- centrifugation Yindlela yokwahlula izinto eziqinileyo kulwelo lobunzima obahlukeneyo kumxube, okoko zangaphambili zingenakunyibilika, kusetyenziswa amandla ojikelezayo okanye amandla e-centrifugal.

Ukwenza oku, isixhobo esibizwa ngokuba yi-centrifuge okanye i-centrifuge sihlala sisetyenziswa, esijikeleza umxube kwi-axis emiselweyo.

Njengoko igama layo lisitsho (icentrifuge: ebaleka embindini), lo mkhosi uthambekele ekutyhaleni ezona zinto zixineneyo ngaphandle kwe-axis yokujikeleza, ushiye ezingaxinananga embindini ngokwawo. Kuchasene namandla e-centripetal.

Bona kwakho: IChromatography

Iindidi zecentrifugation

Umahluko. Ngokusekwe kukwahluka kobunzima bezinto, sisixhobo esisiseko kodwa esingafanelekanga.
Isopychnic. Obu buchule busetyenziswa, umzekelo, ukwahlulahlula amasuntswana anobungakanani obufanayo kodwa noxinano olwahlukeneyo.
Indawo. Umahluko kwinqanaba lentlenge yezinto (ngenxa yobunzima bazo obahlukeneyo) zisetyenziselwa ukwahlulahlula ixesha elinikiweyo le-centrifugation.
Ultracentrifugation. Amandla ayo avumela ukwahlulwa kweemolekyuli kunye nezinto ezingaphantsi kweseli.

Umatshini wokuhlamba. Esi sixhobo sisebenzisa amandla e-centrifugal ukwahlula iimpahla (eziqinileyo) emanzini (ulwelo) ngokusekwe kuxinano lwazo. Kungenxa yoko le nto iimpahla zihlala ziphantse zomile xa zisuswa ngaphakathi.
Icandelo lezobisi. Ubisi luhlulwe phakathi ukwahlula amanzi kunye nomxholo we lipid, kuba le yokugqibela isetyenziselwa ukwenza ibhotolo, okanye ubisi olunamafutha kwintsalela.
Iimoto kwigophe. Xa uqhuba ngokukhawuleza kwigophe lendlela, sihlala siziva sinyanzelwa kukusikhupha endleleni, kude ne-axis yejika. Nguloo mkhosi wamandla.
Ukufumana ii-enzymes. Kwimveliso yezonyango kunye neziyobisi, i-centrifugation ihlala isetyenziselwa ukufumana ii-enzymes ezithile ezivela kwiiseli ezizodwa ezizivelisayo.
Ukwahlulwa kwe-DNA. I-Isopycnic centrifugation ihlala isetyenziswa kwiilebhu zemfuza ukwahlula i-DNA yeselula kwaye ivumele ukuqhubekeka kwayo nokufunda.
Ukutya kweecliacs. Xa kuziwa ekwahluleni iproteni kwi-gluten kukutya okukuyo, inkqubo ye-centrifugation ibalulekile. Yenziwa kuncamathiso lwesitashi, esinomxholo we-gluten ofikelela kwi-8%, kwaye sincitshisiwe saya ngaphantsi kwe-2% kulungelelwaniso olukhethiweyo lokukhetha.
Uvavanyo lwegazi Kusetyenziswa icentrifuge ukwahlulahlula izinto zegazi, ezinjengeplasma nezinye izinto eziqhele ukuxutywa kuyo.
Ukukhawulezisa intlenga. Kwimizi-mveliso eyahlukeneyo yokutya, njengokupheka okanye ukutya okuziinkozo, isenti yokunyusa isantya ikhawulezisa inkqubo yokunyibilikisa eveliswa ngumxhuzulane, ukunciphisa ixesha lokulinda lezinto ezisetyenzisiweyo.
Ukucoca i-latex. Kwishishini le-latex, kufuneka icocwe into leyo umphezulu wayo uhambelana ikakhulu nokunamathela kwamanye amasuntswana, kwaye oku kwenziwa nge-centrifugation, inikwe ubuninzi obuphantsi bento leyo.
Ukomiswa kwezinto eziqinileyo. Esinye isicelo sokusebenza kwecentrifuge kukomiswa kweekristal okanye ezinye izinto ezinemveliso ehamba kunye namanzi. Njengokuba ijikeleza, amanzi ahlukana kwizinto eziqinileyo kwaye alahliwe, eshiya ezo ezifunwayo ngaphandle kolwelo.
Unyango lwelindle. Ukucocwa kwamanzi angcolileyo kuvumela ukukhutshwa kwezinto ezixineneyo ngaphakathi, kungekuphela kwezinto eziqinileyo, kodwa neoyile, amafutha kunye nezinye izinto ezingafunekiyo ezinokuthi zakuba zilahliwe.
Iipaki zokuzonwabisa. Iipaki ezininzi zokuzonwabisa zisebenzisa amandla e-centrifugal ukwenza isiphumo sokukhwela kubakhweli bazo, abajikeleza ngokukhawuleza kwi-axis esisigxina, beqhotyoshelwe ngokuqinileyo esihlalweni esibathintelayo ekubeni baphoswe ngaphandle kwe-axis yokujikeleza.
Abaqhubi bezithuthuthu iPirouette. Umqhubi wesithuthuthu kwindawo engummangaliso yiklasi esekisini, ekwaziyo ukuqhuba eluphahleni lwenqanaba elichaseneyo nomxhuzulane. Oku kuyakwazi ukuyenza emva kokujika okuninzi kwi-axis efanayo ethe tyaba, ukuqokelela isantya kunye nokungenisa kumkhosi we-centrifugal owugcinayo kumbindi wesigaba. Ekugqibeleni lo mkhosi uya kuba mkhulu kangangokuba uya kuba nakho ukuyibeka nkqo intshukumo kunye nokuchasana nomxhuzulane.
Ukuthambekela kwetreyini kaloliwe. Ukuthintela amandla e-centrifugal, imizila kaloliwe ihlala ityibilika kwimijikelo, isenza ukuxhathisa ukuze inganikezeli kumandla ayityhalela ngaphandle kwaye ingaphambuki.
Inguqulelo yasemhlabeni. Isizathu sokuba amandla omxhuzulane elanga angasinyanzeli ukuba singene kuwo kungenxa yamandla e-centrifugal athi, xa ejikeleza kwi-axis ye-king king, ayityhalele ngaphandle, alwe kwaye alinganise umtsalane womxhuzulane.